Orange spotting (OS) disease is one of the emerging diseases of oil palm in Malaysia. The major pathogen in oil palm (OS-CCCVd) is a variant of the coconut cadang-cadang viroid (CCCVd). Even though the disease has not to date significantly affected the oil palm industry, awareness and detection of the disease need to be promoted. A diagnostic molecular tool for the detection of OS-CCCVd has been developed using rapid isothermal amplification that provides for rapid, robust and simple detection of the disease. The presence of the OS-CCCVd disease can be easily determined based on colourimetric changes. Leaves with OS-CCCVd change the reagent mixture to yellowish-green, while the reagent mixture of the negative sample remains orange.