Biomarkers: finding a niche in oil palm tissue culture. Part 2 – targeting the transcriptome

As both embryogenic and non-embryogenic calli were found to be distinctly different, this implies that their gene expression would be differentially regulated as well. Studies in other areas, in particular, medical research have used gene expression patterns to classify and predict the outcomes from specific conditions. This then led to a concerted effort to isolate and characterize the genes related to somatic embryogenesis. Several potential embryogenic markers have been identified, e.g. EgPER1, EgHOX1, EgPK1, EgNAC1, OPSC10 and OPHb1. These genes will then be used for development of a diagnostic screening tool for tissue culture amenity of oil palm.

Memandangkan kedua-dua kalus embriogenik dan bukan embriogenikmempunyai ciri-ciri yang berbeza, ini membayangkan bahawa pengekspresan gen mereka juga dikawal atur secara berbeza. Kajian dalam bidang lain, umpamanya dalam bidang perubatan telah menggunakan pola pengekspresan gen untuk mengklasifikasikan dan meramal hasil sesuatu keadaan yang spesifik tersebut. Ini telah membawa kepada penggemblengan usaha untuk memencil dan mencirikan gen yang terlibat dalam embriogenesis somatik. Beberapa penanda embriogenik yang berpotensi telah dikenal pasti, seperti EgPER1, EgHOX1, EgPK1, EgNAC1, OPSC10 dan OPHb1. Gen ini akan digunakan dalam membangun alat penyaringan diagnostik untuk kemudahan kultur tisu sawit.

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